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Research

Klotho and Anti-Aging

Klotho is a new anti-aging gene whose discovery has had a great impact on aging research. This gene, named after the purported Greek goddess Klotho who spins the thread of life, was so named because of its connection to and direct effects on the aging process. Aging is defined as the age-related decline in physiological functions necessary for survival and fertility. The aging process is multi-factorial, with genetic background and environmental stress as two critical components.  

The klotho gene plays a pivotal role in regulating aging and the development of age-related diseases in mammals. Klotho expression is influenced by many physiological and pathological conditions, and is significantly altered with many physiological processes. Studies have demonstrated that a functional variant of klotho (KL-VS) was associated with human survival and longevity (defined as postnatal life expectancy greater than 75 years). A loss of klotho results in multiple aging-like phenotypes, yet overexpression of klotho gene extends lifespan by 20–30%. It is also interesting to note that klotho gene expresses in limited tissues, but a defect in klotho gene expression leads to multiple aging-like phenotypes involving almost all organ systems in mouse models.

Although genetic mutation of klotho causes multiple aging-related disorders in nearly all organs and tissues, the klotho gene is only expressed in limited tissues in mice, rats and humans: Mouse klotho is predominantly expressed in the kidney and the epithelium of the choroid plexus in the brain. Slight expression of klotho was also found in the pituitary gland, placenta, skeletal muscle, urinary bladder, pancreas, testis, ovary, colon, and inner ear. In the kidney, klotho mRNAs and proteins are localized in the distal tubular cells. Rat klotho is also predominantly expressed in the kidney, and faintly expressed in the brain, lungs, intestines, and gonads. 

Human klotho is primarily expressed in the kidney, although it is also detectable in the placenta, prostate, and small intestines. To date, the expression of the human klotho has been only partly deciphered. Klotho may protect the cardiovascular system by increasing NO production and inhibiting oxidative stress. However, the mechanism mediating the role of klotho in aging-related diseases (e.g., hypertension) remains to be found. Whether the klotho gene delivery can serve as a new therapeutic approach for rejuvenation and aging-related diseases is potentially an interesting topic to pursue, as is the understanding of klotho signaling pathways, which may reveal a new understanding of aging and aging-related diseases.

 

Molecular Mechanisms and Receptors of Klotho

It should be mentioned that the exact biological functions of klotho and its underlying molecular mechanisms are still not fully understood, and these mechanisms remain some of the most intriguing areas in anti-aging research. We hypothesize that perhaps the unknown receptor of klotho is a critical barrier for understanding klotho’s function. Our team believes that it is imperative to clone and characterize receptors that mediate the effect of klotho, and we are designing experiments to investigate klotho gene delivery and the mechanisms which mediate the role of klotho in aging and aging-related diseases.